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KMID : 0379520090250040243
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한국독성학회지
2009 Volume.25 No. 4 p.243 ~ p.251
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Evaluation of Antioxidative Activity of Agrimonia pilosa-Ledeb Leaves on Non-lipid Oxidative Damage
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Hah Dae-Sik
Kim Jong-Shu
Kim Chung-Hui
Kim Eui-Kyung
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Abstract
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Present study was conducted to evaluate the antioxidative activity of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2’-deoxyguanosine (2’-dG) oxidation were investigated. The MeOH extract of the Agrimonia pilosa-Ledeb leaves exhibited high antioxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the Agrimonia pilosa-Ledeb leaves and MeOH extract of the Agrimonia pilosa-Ledeb leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the Agrimonia pilosa-Ledeb leaves on protein damage was observed at 600 μg level (82.05%). The MeOH extracts of the Agrimonia pilosa-Ledeb leaves at 300 ㎍ revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2’-deoxyguanosine (2’-dG) to 8- hydroxy-2-deoxyguanosine (8-OH-2’dG) was inhibited by MeOH extracts of the Agrimonia pilosa- Ledeb leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the Agrimonia pilosa-Ledeb leaves was effective in the protection of non-lipids against various oxidative model systems.
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KEYWORD
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Antioxidative activity, Agrimonia pilosa leaves, 2’-Deoxyguanosine (2’-dG), Non-lipid oxidative model systems
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